1NLT
The crystal structure of Hsp40 Ydj1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.2828,1.2894, 1.244, 1.3237 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 55.025, 55.025, 161.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 3.000 * - 2.700 |
| R-factor | 0.269 |
| Rwork | 0.269 |
| R-free | 0.29600 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.910 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.500 | 2.870 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.054 | 0.145 * |
| Number of reflections | 7827 | |
| <I/σ(I)> | 27.3 | 8.67 |
| Completeness [%] | 95.0 * | 73.1 |
| Redundancy | 5.22 * | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 300 | PEG 4K, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
| 1 | Vapor diffusion * | 7 | 300 | PEG 4K, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | Tris | 100 (mM) | pH7.0 |
| 3 | 1 | reservoir | ethylene glycol | 15 (%) | |
| 4 | 1 | reservoir | PEG4000 | 5 (%) | |
| 5 | 1 | drop | MES | 10 (mM) | pH6.0 |
| 6 | 1 | drop | 150 (mM) |
