1NLC
HIV-1 DIS(Mal) duplex Zn-soaked
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.26 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 59.333, 59.333, 63.683 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.850 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.21900 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 9.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.040 * | 0.195 * |
| Number of reflections | 21043 | |
| <I/σ(I)> | 32.3 | |
| Completeness [%] | 95.2 * | 69.3 * |
| Redundancy | 3.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 310 | MPD, Cacodylate, KCl, MgCl2, spermine, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sermine | 50 (mM) | |
| 2 | 1 | reservoir | MPD | 10 (%) | |
| 3 | 1 | drop | MPD | 50 (%) | |
| 4 | 1 | drop | sodium cacodylate | 50 (mM) | |
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | drop | 300 (mM) |
