1NLB
crystal structure of anti-HCV monoclonal antibody 19D9D6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-11 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.480, 173.680, 41.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.197 |
| Rwork | 0.181 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1N64 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.450 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.080 * | 0.220 * |
| Total number of observations | 47973 * | |
| Number of reflections | 42445 | |
| Completeness [%] | 79.5 | 42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 9 | 290 | Stura, E.A., (2001) J. Crystal Growth, 232, 545. * |
