1NL5
Engineered High-affinity Maltose-Binding Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 121.590, 121.590, 62.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.800 - 2.100 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1anf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.100 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.800 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.046 * | 0.190 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 * | PEG 3350, Zinc Chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 100K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG5000 MME | 18-24 (%) | |
| 2 | 1 | reservoir | cacodylate | 0.1 (M) | pH6.2 |
| 3 | 1 | reservoir | sodium acetate | 200 (mM) | |
| 4 | 1 | reservoir | zinc acetate | 10 (mM) | |
| 5 | 1 | reservoir | 5 (mM) | ||
| 6 | 1 | reservoir | maltose | 1 (mM) |
