1NKZ
Crystal structure of LH2 B800-850 from Rps. acidophila at 2.0 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-06-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 117.052, 117.052, 298.438 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.1704 |
| Rwork | 0.169 |
| R-free | 0.19400 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1kzu |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.620 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | DM |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 * | 0.306 |
| Number of reflections | 48976 | 2431 * |
| <I/σ(I)> | 8.7 | 2.5 |
| Completeness [%] | 99.3 * | 95 |
| Redundancy | 4.1 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 9.5 * | 20 * | Papiz, M.Z., (1989) J. Mol. Biol., 209, 833. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | phosphate | 0.9 (M) | |
| 2 | 1 | drop | benzamidine hydrochloride | 3 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.4 (M) |
