1NKZ

Crystal structure of LH2 B800-850 from Rps. acidophila at 2.0 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	1997-06-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.87
Spacegroup name	H 3 2
Unit cell lengths	117.052, 117.052, 298.438
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.1704
Rwork	0.169
R-free	0.19400 *
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1kzu
RMSD bond length	0.019
RMSD bond angle	1.620 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	DM
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.050 *	0.306
Number of reflections	48976	2431 *
<I/σ(I)>	8.7	2.5
Completeness [%]	99.3 *	95
Redundancy	4.1	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	9.5 *	20 *	Papiz, M.Z., (1989) J. Mol. Biol., 209, 833. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	phosphate	0.9 (M)
2	1	drop	benzamidine hydrochloride	3 (%)
3	1	reservoir	ammonium sulfate	2.4 (M)