1NKR

INHIBITORY RECEPTOR (P58-CL42) FOR HUMAN NATURAL KILLER CELLS

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	90
Detector technology	CCD
Collection date	1996-10
Detector	PRINCETON 2K
Spacegroup name	P 61
Unit cell lengths	92.360, 92.360, 46.770
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.700
R-factor	0.205
Rwork	0.227
R-free	0.25500
Structure solution method	MAD
RMSD bond length	0.010 *
RMSD bond angle	1.830 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.073	0.234
Number of reflections	25065
<I/σ(I)>	17.1	4
Completeness [%]	99.5	97.8
Redundancy	7	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.7		PROTEIN WAS CRYSTALLIZED FROM 0.55 M (NH4)2HPO4, 50 MM SODIUM CITRATE, PH 5.4, FINAL PH 7.7. CRYSTALS WERE HARVESTED IN 1.5 M (NH4)2HPO4, 50 MM SODIUM CITRATE, PH 5.4, FINAL PH 7.7; THEN SOAKED IN 1.5 M (NH4)2HPO4, 50 MM SODIUM CITRATE, PH 5.4, 25% GLYCEROL, FINAL PH 7.7, AND FLASH-COOLED WITH LIQUID NITROGEN.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium phosphate	0.55 (M)
2	1	1	sodium citrate	50 (mM)