1NKO

Energetic and structural basis of sialylated oligosaccharide recognition by the natural killer cell inhibitory receptor p75/AIRM1 or Siglec-7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	32.461, 49.715, 39.787
Unit cell angles	90.00, 113.08, 90.00

Refinement procedure

Resolution	15.000 * - 1.450
R-factor	0.277
Rwork	0.277
R-free	0.21800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qfo
RMSD bond length	0.010 *
RMSD bond angle	1.640 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	15.000
High resolution limit [Å]	1.450	1.450
Rmerge	0.060 *	0.388 *
Total number of observations	238401 *
Number of reflections	20819 *
Completeness [%]	89.3 *	68.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *	277 *	Dimasi, N., (2003) Acta Crystallogr., D59, 1856. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	drop	MES	25 (mM)	pH5.5
3	1	reservoir	PEG8000	20 (%(w/v))
4	1	reservoir		0.2 (M)
5	1	reservoir	phosphate-citrate	0.1 (M)	pH4.2