1NKD
ATOMIC RESOLUTION (1.07 ANGSTROMS) STRUCTURE OF THE ROP MUTANT <2AA>
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 47.060, 37.880, 31.650 |
| Unit cell angles | 90.00, 100.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.090 |
| R-factor | 0.104 |
| R-free | 0.13400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.063 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXL-93 |
| Refinement software | SHELXL-93 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.130 | 1.190 |
| High resolution limit [Å] | 1.090 | 1.090 |
| Rmerge | 0.450 | 0.197 |
| Number of reflections | 22361 | |
| <I/σ(I)> | 18.9 | 8.2 |
| Completeness [%] | 98.2 | 97.5 |
| Redundancy | 3.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 100 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 0.8 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.6 (M) |
