1NJH

Crystal Structure of Bacillus subtilis YojF protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-18
Detector	SBC-2
Wavelength(s)	0.97921
Spacegroup name	P 3 2 1
Unit cell lengths	78.703, 78.703, 31.074
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.280 - 1.700
R-factor	0.223
Rwork	0.220
R-free	0.25300
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	d*TREK
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.270	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.042	0.513
Number of reflections	12326
<I/σ(I)>	9.4	2.4
Completeness [%]	99.2	100
Redundancy	6.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	Isopropanol, Sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	Isopropanol, Sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	Isopropanol, Sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K