1NJG
Nucleotide-free form of an Isolated E. coli Clamp Loader Gamma Subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.335, 85.471, 56.932 |
| Unit cell angles | 90.00, 93.15, 90.00 |
Refinement procedure
| Resolution | 56.850 - 2.200 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.26500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jr3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.477 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.040 | 0.338 |
| Total number of observations | 255606 * | |
| Number of reflections | 23273 * | |
| <I/σ(I)> | 20.9 | 2.8 |
| Completeness [%] | 92.1 * | 79 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | 293 | PEG 3350, ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mM) | |
| 2 | 1 | drop | AMP-PNP | 12 (mM) | |
| 3 | 1 | drop | 27 (mM) | ||
| 4 | 1 | reservoir | PEG3350 | 12-19 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 0.12-0.19 (M) |
