1NJG

Nucleotide-free form of an Isolated E. coli Clamp Loader Gamma Subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-27
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	47.335, 85.471, 56.932
Unit cell angles	90.00, 93.15, 90.00

Refinement procedure

Resolution	56.850 - 2.200
R-factor	0.238
Rwork	0.238
R-free	0.26500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jr3
RMSD bond length	0.008
RMSD bond angle	1.477 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.040	0.338
Total number of observations	255606 *
Number of reflections	23273 *
<I/σ(I)>	20.9	2.8
Completeness [%]	92.1 *	79
Redundancy	3	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6	293	PEG 3350, ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mM)
2	1	drop	AMP-PNP	12 (mM)
3	1	drop		27 (mM)
4	1	reservoir	PEG3350	12-19 (%)
5	1	reservoir	ammonium sulfate	0.12-0.19 (M)