1NJ2

Crystal structure of Prolyl-tRNA Synthetase from Methanothermobacter thermautotrophicus

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (3.11 Å)

Cell axes145.077145.077170.359
Cell angles90.0090.00120.00
SpacegroupP 65 2 2
Resolution limits29.95 - 3.11
the highest resolution shell value3.290 - 3.110
R-factor0.239
R-work0.23900
the highest resolution shell value0.366
R-free0.27300
the highest resolution shell value0.387
RMSD bond length0.009
RMSD bond angle1.400 (22.900*)

Data Collection Statistics

Resolution limits30.00 - 3.10
the highest resolution shell value -
Number of reflections35918
Rmerge_l_obs0.066
the highest resolution shell value0.770
Completeness99.6
the highest resolution shell value99.7*
Redundancy4.7
I/sigma(I)-3

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1VAPOR DIFFUSION, HANGING DROP (Vapor diffusion*)7.5 (8.5*)293 (12-25*)*

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein5 (mg/ml)
21dropTris20 (mM)pH8.5
31drop200 (mM)
41drop2-mercaptoethanol5 (mM)
51reservoirammonium sulfate0.2 (M)
61reservoirPEG400020-30 (%)
71reservoir2-mercaptoethanol5 (mM)
81reservoir0.1 (M)pH4.5
Annotated Information is extracted from Literature Info*