1NIV
MANNOSE-SPECIFIC AGGLUTININ (LECTIN) FROM SNOWDROP (GALANTHUS NIVALIS) BULBS IN COMPLEX WITH MANNOSE-ALPHA 1,3-METHYL-D-MANNOSE
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1994-09-09 |
Detector | RIGAKU |
Spacegroup name | I 21 3 |
Unit cell lengths | 138.149, 138.149, 138.149 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 3.000 |
R-factor | 0.226 * |
Rwork | 0.227 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | GNA-ALPHA-MEMAN COMPLEX |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 3.110 | |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.107 | 0.289 |
Total number of observations | 39125 * | |
Number of reflections | 8280 | |
<I/σ(I)> | 9 | 2.9 |
Completeness [%] | 93.1 | 86.3 |
Redundancy | 4.7 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 8.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 25 (mg/ml) | |
2 | 1 | 1 | ammonium bicarbonate | 0.05 (M) | |
3 | 1 | 1 | MeMan-2 | 0.023 (M) |