1NIK
Wild Type RNA Polymerase II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-31 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 224.098, 394.462, 284.343 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 4.100 |
R-factor | 0.3335 |
Rwork | 0.334 |
R-free | 0.35700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1I6H WITH DNA AND RNA REMOVED 1GO3 COVERTED TO POLY-ALA MODEL |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 4.250 |
High resolution limit [Å] | 4.100 | 4.100 |
Rmerge | 0.108 * | 0.614 * |
Number of reflections | 96820 * | 9357 * |
<I/σ(I)> | 5.9 | 1.06 |
Completeness [%] | 98.8 | 96.6 |
Redundancy | 3.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 291 | peg400, peg 6K, NaCl, Dioaxne, ZnCl2, Ammonium phosphate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 6.30 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
10 | 1 | reservoir | PEG400 | 20-25 (%) | |
2 | 1 | drop | Tris-Cl | 5 (mM) | pH7.5 |
3 | 1 | drop | ammonium sulfate | 60 (mM) | |
4 | 1 | drop | dithiothreitol | 10 (mM) | |
5 | 1 | reservoir | ammonium phosphate | 100 (mM) | pH6.3 |
6 | 1 | reservoir | 100 (mM) | ||
7 | 1 | reservoir | dioxane | 5 (mM) | |
8 | 1 | reservoir | 1 (mM) | ||
9 | 1 | reservoir | PEG6000 | 5 (%) |