1NHY
Crystal Structure of the GST-like Domain of Elongation Factor 1-gamma from Saccharomyces cerevisiae.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-24 |
Detector | ADSC |
Wavelength(s) | 0.9792 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 164.520, 164.520, 164.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.2349 |
Rwork | 0.235 |
R-free | 0.23490 |
Structure solution method | SAD |
Starting model (for MR) | None |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXD |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 3.170 |
High resolution limit [Å] | 3.000 | 3.000 * |
Rmerge | 0.053 | 0.362 |
Number of reflections | 14125 | |
<I/σ(I)> | 50.1 | |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 10.7 | 0.109 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 * | 6 * | Ammonium sulfate, EDTA, DTT, sodium azide, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 279K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2.36-2.4 (M) | |
2 | 1 | reservoir | GSH | 2 (mM) | |
3 | 1 | reservoir | dithiothreitol | 3 (mM) | |
4 | 1 | reservoir | 1 (mM) | ||
5 | 1 | reservoir | EDTA-NaOH | 0.1 (mM) | pH7.5 |
6 | 1 | reservoir | MES- NaOH | 25 (mM) | pH6. |
7 | 1 | reservoir | HEPES-NaOH | 25 (mM) | pH7. |
8 | 1 | drop | protein | 8.4 (mg/ml) |