1NHY
Crystal Structure of the GST-like Domain of Elongation Factor 1-gamma from Saccharomyces cerevisiae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-24 |
| Detector | ADSC |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 164.520, 164.520, 164.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.2349 |
| Rwork | 0.235 |
| R-free | 0.23490 |
| Structure solution method | SAD |
| Starting model (for MR) | None |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 3.170 |
| High resolution limit [Å] | 3.000 | 3.000 * |
| Rmerge | 0.053 | 0.362 |
| Number of reflections | 14125 | |
| <I/σ(I)> | 50.1 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 10.7 | 0.109 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 * | 6 * | Ammonium sulfate, EDTA, DTT, sodium azide, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 279K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.36-2.4 (M) | |
| 2 | 1 | reservoir | GSH | 2 (mM) | |
| 3 | 1 | reservoir | dithiothreitol | 3 (mM) | |
| 4 | 1 | reservoir | 1 (mM) | ||
| 5 | 1 | reservoir | EDTA-NaOH | 0.1 (mM) | pH7.5 |
| 6 | 1 | reservoir | MES- NaOH | 25 (mM) | pH6. |
| 7 | 1 | reservoir | HEPES-NaOH | 25 (mM) | pH7. |
| 8 | 1 | drop | protein | 8.4 (mg/ml) |
