1NHR
AN L40C MUTATION CONVERTS THE CYSTEINE-SULFENIC ACID REDOX CENTRE IN ENTEROCOCCAL NADH PEROXIDASE TO A DISULFIDE
Experimental procedure
Spacegroup name | I 2 2 2 |
Unit cell lengths | 77.700, 134.600, 145.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.100 |
R-factor | 0.17 |
Rwork | 0.170 |
RMSD bond length | 0.011 * |
RMSD bond angle | 2.600 * |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.100 * | 2.000 * |
Rmerge | 0.057 * | |
Number of reflections | 44912 | |
Completeness [%] | 91.7 | 80 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 25 (mM) | |
3 | 1 | drop | EDTA | 0.3 (mM) | |
4 | 1 | drop | DTT | 2 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 1.9 (M) | |
6 | 1 | reservoir | FAD | 0.005 (mM) | |
7 | 1 | reservoir | potassium phosphate | 0.1 (M) |