1NHK
CRYSTAL STRUCTURE OF MYXOCOCCUS XANTHUS NUCLEOSIDE DIPHOSPHATE KINASE AND ITS INTERACTION WITH A NUCLEOTIDE SUBSTRATE AT 2.0 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.600, 63.600, 158.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.20600 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 22.400 * |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 * | |
| High resolution limit [Å] | 1.900 * | 1.900 * |
| Rmerge | 0.052 * | 0.206 * |
| Total number of observations | 113713 * | |
| Number of reflections | 26296 | |
| Completeness [%] | 99.1 | |
| Redundancy | 4.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.7 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | nucleoside diphosphate kinase | 24 (mg/ml) | |
| 10 | 1 | reservoir | sodium acetate | 250 (mM) | |
| 2 | 1 | drop | cAMP | 8 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | Tris-HCl | 10 (mM) | |
| 5 | 1 | drop | PEG3000 | 13 (%) | |
| 6 | 1 | drop | acetic acid | 50 (mM) | |
| 7 | 1 | drop | sodium acetate | 250 (mM) | |
| 8 | 1 | reservoir | PEG3000 | 18 (%) | |
| 9 | 1 | reservoir | acetic acid | 50 (mM) |
