1NHI
Crystal structure of N-terminal 40KD MutL (LN40) complex with ADPnP and one potassium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 98 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 61.897, 71.415, 190.342 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.2081 |
| Rwork | 0.208 |
| R-free | 0.22900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b63 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.117 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.043 * | 0.139 * |
| Number of reflections | 51448 | |
| Completeness [%] | 95.6 * | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.2 | 20 * | Ban, C., (1999) Cell(Cambridge,Mass.), 97, 85. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 10 | 1 | reservoir | PEG4000 | 24 (%(w/v)) | |
| 11 | 1 | reservoir | 5 (mM) | ||
| 12 | 1 | reservoir | EDTA | 1 (mM) | |
| 13 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | EDTA | 1.0 (mM) | |
| 5 | 1 | drop | 150 (mM) | ||
| 6 | 1 | drop | 5 (mM) | ||
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.2 |
| 8 | 1 | reservoir | ammonium sulfate | 150 (mM) | |
| 9 | 1 | reservoir | dithiothreitol | 50 (mM) |
