1NH9
Crystal Structure of a DNA Binding Protein Mja10b from the hyperthermophile Methanococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-15 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9868 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 51.506, 51.506, 125.307 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| Rwork | 0.209 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h0x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.405 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.049 * | 0.311 * |
| Total number of observations | 91002 * | |
| Number of reflections | 6946 * | |
| <I/σ(I)> | 21.4 | 4.2 |
| Completeness [%] | 96.6 * | 99.1 |
| Redundancy | 13.1 * | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.25 | 291 | Wang, G.G., (2002) Acta Cryst., D58, 1240. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 40 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.25 |
