1NH8
ATP PHOSPHORIBOSYLTRANSFERASE (ATP-PRTASE) FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH AMP AND HISTIDINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-08-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 113.754, 113.754, 124.254 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.19946 |
Rwork | 0.198 |
R-free | 0.23600 * |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.500 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.700 | 1.846 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.047 * | 0.134 * |
Number of reflections | 37740 | |
Completeness [%] | 97.5 * | 99.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 16 * | Lithium sulfate, Ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | sodium citrate | 0.1 (M) | pH5.6 |
2 | 1 | drop | ammonium sulfate | 0.5 (M) | |
3 | 1 | drop | lithium sulfate | 1.0 (M) | |
4 | 1 | reservoir | AMP | 5 (mM) | |
5 | 1 | reservoir | histidine | 0.1 (mM) |