1NH8
ATP PHOSPHORIBOSYLTRANSFERASE (ATP-PRTASE) FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH AMP AND HISTIDINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 113.754, 113.754, 124.254 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.19946 |
| Rwork | 0.198 |
| R-free | 0.23600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.700 | 1.846 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.047 * | 0.134 * |
| Number of reflections | 37740 | |
| Completeness [%] | 97.5 * | 99.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 16 * | Lithium sulfate, Ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium citrate | 0.1 (M) | pH5.6 |
| 2 | 1 | drop | ammonium sulfate | 0.5 (M) | |
| 3 | 1 | drop | lithium sulfate | 1.0 (M) | |
| 4 | 1 | reservoir | AMP | 5 (mM) | |
| 5 | 1 | reservoir | histidine | 0.1 (mM) |
