1NH2

Crystal structure of a yeast TFIIA/TBP/DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	1997-03-21
Detector	MARRESEARCH
Wavelength(s)	0.9076
Spacegroup name	P 21 21 21
Unit cell lengths	59.010, 92.020, 117.017
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.900
R-factor	0.201
Rwork	0.201
R-free	0.23400 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.900	1.900 *
Rmerge	0.056 *	0.147 *
Total number of observations	217906 *
Number of reflections	48353 *
Completeness [%]	94.9 *	98.9 *
Redundancy	4.5 *	4.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
10	1	reservoir	dithiothreitol	1 (mM)
11	1	reservoir	PEG6000	12 (%(w/v))
2	1	drop	Bis-Tris	2 (mM)	pH7.0
3	1	drop		20 (mM)
4	1	drop	EDTA	0.1 (mM)
5	1	drop	dithiothreitol	0.5 (mM)
6	1	drop		0.01 (%(w/v))
7	1	reservoir	bis-Tris	50 (mM)	pH6.5
8	1	reservoir		100 (mM)
9	1	reservoir		10 (mM)