1NH2
Crystal structure of a yeast TFIIA/TBP/DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 1997-03-21 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9076 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.010, 92.020, 117.017 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.900 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.23400 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 1.900 | 1.900 * |
Rmerge | 0.056 * | 0.147 * |
Total number of observations | 217906 * | |
Number of reflections | 48353 * | |
Completeness [%] | 94.9 * | 98.9 * |
Redundancy | 4.5 * | 4.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | 22 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
10 | 1 | reservoir | dithiothreitol | 1 (mM) | |
11 | 1 | reservoir | PEG6000 | 12 (%(w/v)) | |
2 | 1 | drop | Bis-Tris | 2 (mM) | pH7.0 |
3 | 1 | drop | 20 (mM) | ||
4 | 1 | drop | EDTA | 0.1 (mM) | |
5 | 1 | drop | dithiothreitol | 0.5 (mM) | |
6 | 1 | drop | 0.01 (%(w/v)) | ||
7 | 1 | reservoir | bis-Tris | 50 (mM) | pH6.5 |
8 | 1 | reservoir | 100 (mM) | ||
9 | 1 | reservoir | 10 (mM) |