1NH1
Crystal Structure of the Type III Effector AvrB from Pseudomonas syringae.
Experimental procedure
Experimental method | MAD |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 200 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU |
Spacegroup name | P 65 |
Unit cell lengths | 125.028, 125.028, 63.246 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 500.000 * - 2.200 |
Rwork | 0.222 |
R-free | 0.27500 * |
Structure solution method | MAD |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.540 * |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.200 | |
Rmerge | 0.066 * | 0.493 * |
Number of reflections | 27892 | |
Completeness [%] | 93.4 * | 64.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 * | 4 * | VAPOR DIFFUSION, HANGING DROP |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 * | 4 * | VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 20 (mM) | pH7.9 |
2 | 1 | drop | 50 (mM) | ||
3 | 1 | drop | TCEP | 0.25 (mM) | |
4 | 1 | drop | protein | 9.5 (mg/ml) | |
5 | 1 | reservoir | PEG600 | 40 (%) | |
6 | 1 | reservoir | CHES | 100 (mM) | pH9.5-10.0 |