1NGX
Chimeric Germline Fab 7g12 with jeffamine fragment bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.666, 64.723, 93.382 |
| Unit cell angles | 90.00, 92.58, 90.00 |
Refinement procedure
| Resolution | 19.870 - 1.800 |
| Rwork | 0.225 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.420 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 87547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | PEG2000MME, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 100K |
