1NGM
Crystal structure of a yeast Brf1-TBP-DNA ternary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-07-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.100, 0.919425, 0.918969 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 93.610, 152.600, 256.638 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.500 * - 2.950 |
R-factor | 0.278 |
Rwork | 0.276 |
R-free | 0.30800 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.441 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.060 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.062 | |
Total number of observations | 269787 * | |
Number of reflections | 74859 * | |
Completeness [%] | 95.5 * | 87 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4. * | MES, NaCl, BME, glycerol, MgCl2, PEG2000MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MES | ||
11 | 1 | 2 | MgCl2 | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | BME | ||
4 | 1 | 1 | glycerol | ||
5 | 1 | 1 | MgCl2 | ||
6 | 1 | 1 | PEG2000MME | ||
8 | 1 | 2 | NaCl |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 50 (mM) | pH6.5 | |
2 | 1 | reservoir | 100 (mM) | or LiCl | |
3 | 1 | reservoir | 10 (mM) | ||
4 | 1 | reservoir | 2-mercaptoethanol | 2 (mM) | |
5 | 1 | reservoir | glycerol | 25 (%(v/v)) | |
6 | 1 | reservoir | PEG2000 MME | 10 (%(v/v)) |