1NFF

Crystal structure of Rv2002 gene product from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	70.377, 70.377, 148.934
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 1.800
R-factor	0.164
Rwork	0.164
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.290 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.063 *	0.345 *
Total number of observations	280336 *
Number of reflections	40259
Completeness [%]	99.7	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	296	PEG 3000, calcium acetate, Tris-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.5 (M)
2	1	reservoir	PEG400	5.0 (%(v/v))
3	1	reservoir	sodium HEPES	100 (mM)	pH7.5
4	1	drop	protein	14.4 (mg/ml)
5	1	drop	Tris-HCl	20 (mM)	pH8.0
6	1	drop	PMSF	0.1 (mM)
7	1	drop	NAD+	2 (mM)