1NFF
Crystal structure of Rv2002 gene product from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 70.377, 70.377, 148.934 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 * - 1.800 |
R-factor | 0.164 |
Rwork | 0.164 |
R-free | 0.19900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.290 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.063 * | 0.345 * |
Total number of observations | 280336 * | |
Number of reflections | 40259 | |
Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 296 | PEG 3000, calcium acetate, Tris-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
2 | 1 | reservoir | PEG400 | 5.0 (%(v/v)) | |
3 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.5 |
4 | 1 | drop | protein | 14.4 (mg/ml) | |
5 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
6 | 1 | drop | PMSF | 0.1 (mM) | |
7 | 1 | drop | NAD+ | 2 (mM) |