1NF9
Crystal Structure of PhzD protein from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-09-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.850, 77.030, 82.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.156 |
| Rwork | 0.155 |
| R-free | 0.22500 |
| Structure solution method | SAD S |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.031 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.032 | 0.097 |
| Total number of observations | 357616 * | |
| Number of reflections | 33819 | |
| <I/σ(I)> | 25.6 | 3.2 |
| Completeness [%] | 92.7 | 80.4 |
| Redundancy | 11 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 298 | 10-20% polyethylene glycol 4000, 0.2M ammonium formate, 0.2% beta-octylglucoside, 1mM aminodeoxychorismate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MOPS | 50 (mM) | |
| 2 | 1 | drop | dithiothreitol | 1 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | pH7.2 |
| 4 | 1 | drop | protein | 17 (mg/ml) | |
| 5 | 1 | reservoir | beta-octylglucoside | 0.2 (%) |
