1NEX

Crystal Structure of ScSkp1-ScCdc4-CPD peptide complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	110
Detector technology	CCD
Collection date	2002-03-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9798, 0.9800
Spacegroup name	P 32
Unit cell lengths	107.669, 107.669, 168.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.700
R-factor	0.239
Rwork	0.239
R-free	0.27300
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.420 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.050 *	0.289 *
Total number of observations	298371 *
Number of reflections	116218 *
Completeness [%]	97.7 *	93.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	20 *	Ammonium Sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	20 *	Ammonium Sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	HEPES	10 (mM)	pH7.5
3	1	drop		250 (mM)
4	1	drop	dithiothreitol	1 (mM)
5	1	reservoir	Tris	0.1 (M)	pH8.5
6	1	reservoir	ammonium sulfate	1.5 (M)