1NEU

STRUCTURE OF MYELIN MEMBRANE ADHESION MOLECULE P0

Experimental procedure

Spacegroup name	I 4 2 2
Unit cell lengths	88.900, 88.900, 91.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.215
Rwork	0.215
R-free	0.26800
Structure solution method	MAD, SIR/AS
RMSD bond length	0.014
RMSD bond angle	1.541
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.041 *
Number of reflections	14552 *
Completeness [%]	98.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	45 (mg/ml)
2	1	drop	Tris	10 (mM)
3	1	drop		0.3 (M)
4	1	reservoir	Tris	10 (mM)
5	1	reservoir		1.0 (M)