1NEN

Complex II (Succinate Dehydrogenase) From E. Coli with Dinitrophenol-17 inhibitor co-crystallized at the ubiquinone binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-07
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9150
Spacegroup name	H 3 2
Unit cell lengths	138.800, 138.800, 521.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.900
R-factor	0.271
Rwork	0.271
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	21.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.960
High resolution limit [Å]	2.900	2.900
Rmerge	0.116 *	0.428 *
Total number of observations	119504 *
Number of reflections	42735
Completeness [%]	98.1	96.6
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	20 *	Tris-HCl, CaCl2, PEG 400, BaCl2, ethylene glycol, DNP17, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	0.1 (M)	pH8.2
2	1	reservoir		0.2 (M)
3	1	reservoir	PEG400	24-29 (%)
4	1	reservoir		0.01 (M)
5	1	reservoir	ethylene glycol	3 (%)