1NEK

Complex II (Succinate Dehydrogenase) From E. Coli with ubiquinone bound

Experimental procedure

実験手法	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-08
Detector	MARRESEARCH
Wavelength(s)	1.0081
Spacegroup name	H 3 2
格子定数 [Å]	138.800, 138.800, 521.900
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	40.000 - 2.600
R因子	0.247
Rwork	0.247
R-free	0.28900
Structure solution method	MAD
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	40.000	2.690
分解能 [Å] (高)	2.600	2.600
独立反射数	53727
完全性 [%]	89.9	90
冗長性	2.9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	8.2	293	Tris-HCl, CaCl2, PEG 400, BaCl2, ethylene glycol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K