1NED
CRYSTAL STRUCTURE OF HSLV (CLPQ) AT 3.8 ANGSTROMS RESOLUTION
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 108.400, 108.400, 103.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.800 |
| R-factor | 0.256 |
| Rwork | 0.256 |
| R-free | 0.31500 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 24.900 * |
| Data reduction software | MOSFLM (V. 5.3) |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.842) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.930 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.073 * | |
| Total number of observations | 36796 * | |
| Number of reflections | 6404 | |
| <I/σ(I)> | 3.8 | |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 5.7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | 100MM HEPES/NAOH PH 7.5 200MM SODIUM ACETATE 0.02 % SODIUM AZIDE 9-14% ETHANOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES-NaOH | 100 (mM) | pH7.5 |
| 2 | 1 | reservoir | sodium acetate | 200 (mM) | |
| 3 | 1 | reservoir | 0.02 (%) | ||
| 4 | 1 | reservoir | ethanol | 9-14 (%) | |
| 5 | 1 | drop | protain | 10 (mg/ml) | |
| 6 | 1 | drop | Tris-HCl | 20 (mM) | |
| 7 | 1 | drop | 300 (mM) | ||
| 8 | 1 | drop | EDTA | 1 (mM) | |
| 9 | 1 | drop | 1 (mM) |
