1NE9

Crystal Structure of Weissella viridescens FemX at 1.7 Ang Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-14
Detector	MARRESEARCH
Wavelength(s)	0.97976
Spacegroup name	P 1 21 1
Unit cell lengths	42.030, 99.920, 45.840
Unit cell angles	90.00, 116.02, 90.00

Refinement procedure

Resolution	35.000 * - 1.700
R-factor	0.176
Rwork	0.176
R-free	0.20900
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	23.200 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000 *	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.044 *	0.139 *
Total number of observations	156408 *
Number of reflections	36921
<I/σ(I)>	10.2	3.7
Completeness [%]	98.8	97.7
Redundancy	4.2	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	298	PEG 6K, NaCl, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG6000	30 (%)
3	1	reservoir		300 (mM)
4	1	reservoir	cacodylate	100 (mM)	pH6.5