1NDM

Crystal structure of Fab fragment of antibody HyHEL-26 complexed with lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Detector technology	IMAGE PLATE
Spacegroup name	P 21 21 2
Unit cell lengths	90.680, 164.020, 40.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 * - 2.100
R-factor	0.2081
Rwork	0.208
R-free	0.26200 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006 *
RMSD bond angle	26.400 *
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.100	2.000 *
Rmerge	0.086 *	0.208 *
Total number of observations	84092 *
Number of reflections	31484
Completeness [%]	90.0 *	82.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5 *		VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	reservoir	PEG4000	14 (%(w/v))
3	1	reservoir	ammonium acetate	100 (mM)
4	1	reservoir	sodium acetate	50 (mM)	pH5.0