1NDJ

Streptavidin Mutant Y43F with Biotin at 1.81A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1998-04-18
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	51.300, 98.400, 52.800
Unit cell angles	90.00, 112.90, 90.00

Refinement procedure

Resolution	10.000 - 1.800 *
R-factor	0.2273
Rwork	0.222
R-free	0.31200
Structure solution method	ISOMORPHOUS
Starting model (for MR)	1swe
RMSD bond length	0.006
RMSD bond angle	0.022
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000
High resolution limit [Å]	1.800 *
Rmerge	0.070 *	0.320 *
Number of reflections	35211
Completeness [%]	80.0 *	30 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	293	PEG4000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	reservoir	PEG4000	16 (%)
3	1	reservoir	HEPES	0.1 (M)	pH7.5