1NDH
CRYSTAL STRUCTURE OF NADH-CYTOCHROME B5 REDUCTASE FROM PIG LIVER AT 2.4 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.900, 73.100, 48.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.100 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.300 |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Rmerge | 0.055 * |
| Number of reflections | 18695 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4 or 20 * | Miki, K., (1987) J. Biol. Chem., 262, 11801. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-40 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 3-20 (%(w/v)) | |
| 3 | 1 | reservoir | potassium phosphate | 50 (mM) | |
| 4 | 1 | reservoir | EDTA | 0.1 (mM) | |
| 5 | 1 | reservoir | Triton N-101 | 0.1 (%) | |
| 6 | 1 | reservoir | glycerol | 10 (%) |
