1NDG

Crystal structure of Fab fragment of antibody HyHEL-8 complexed with its antigen lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Detector technology	CCD
Spacegroup name	P 42 21 2
Unit cell lengths	89.870, 89.870, 148.963
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 * - 1.900
R-factor	0.2061
Rwork	0.206
R-free	0.25400 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.400 *
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.320
High resolution limit [Å]	1.900	1.800 *
Rmerge	0.053 *	0.300 *
Total number of observations	403172 *
Number of reflections	46465 *
Completeness [%]	95.0 *	80.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	reservoir	PEG8000	20 (%(w/v))
3	1	reservoir		50 (mM)
4	1	reservoir	sodium acetate	100 (mM)	pH4.6