1ND4
Crystal structure of aminoglycoside-3'-phosphotransferase-IIa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.048, 101.893, 71.972 |
| Unit cell angles | 90.00, 105.58, 90.00 |
Refinement procedure
| Resolution | 34.000 - 2.100 |
| Rwork | 0.206 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 4.500 * | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.088 * | 0.415 * |
| Completeness [%] | 99.4 * | 99.7 |
| Redundancy | 9.7 * | 8.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 4 * | 100mM cacodylate, 150mM magnesium acetate, 20-26% PEG8000, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 10 (mg/ml) | |
| 2 | 1 | drop | MES | 40 (mM) | pH6.4 |
| 3 | 1 | reservoir | cacodylate | 100 (mM) | pH6.4 |
| 4 | 1 | reservoir | magnesium acetate | 150 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 20-26 (%) |
