1NCJ
N-CADHERIN, TWO-DOMAIN FRAGMENT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01 |
| Detector | FUJI |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 99.000, 99.000, 136.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 3.400 |
| R-factor | 0.212 * |
| Rwork | 0.212 |
| R-free | 0.32100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1NCG AND 1EDH |
| RMSD bond length | 0.013 |
| RMSD bond angle | 27.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.930 | 0.322 |
| Number of reflections | 9451 | |
| <I/σ(I)> | 12.96 | 3.12 |
| Completeness [%] | 98.6 | 97.7 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5 | drop contains equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | Na acetate | 50 (mM) | |
| 3 | 1 | reservoir | 15 (mM) | ||
| 4 | 1 | reservoir | uranyl acetate | 1 (mM) |
