1NCJ

N-CADHERIN, TWO-DOMAIN FRAGMENT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-01
Detector	FUJI
Spacegroup name	I 4 2 2
Unit cell lengths	99.000, 99.000, 136.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 3.400
R-factor	0.212 *
Rwork	0.212
R-free	0.32100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1NCG AND 1EDH
RMSD bond length	0.013
RMSD bond angle	27.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.500
High resolution limit [Å]	3.400	3.400
Rmerge	0.930	0.322
Number of reflections	9451
<I/σ(I)>	12.96	3.12
Completeness [%]	98.6	97.7
Redundancy	4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5		drop contains equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	Na acetate	50 (mM)
3	1	reservoir		15 (mM)
4	1	reservoir	uranyl acetate	1 (mM)