1NBX
Streptavidin Mutant Y43A at 1.70A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-04-29 |
Detector | RIGAKU RAXIS II |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.300, 87.700, 46.800 |
Unit cell angles | 90.00, 98.90, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.700 |
R-factor | 0.2109 |
Rwork | 0.207 |
R-free | 0.27300 * |
Structure solution method | isomorphous |
Starting model (for MR) | 1swa |
RMSD bond length | 0.007 |
RMSD bond angle | 0.027 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 1.700 | |
Rmerge | 0.060 * | 0.410 * |
Number of reflections | 43769 * | |
Completeness [%] | 86.0 * | 35 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 | 293 | MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20-30 (mg/ml) | |
2 | 1 | reservoir | MPD | 50 (%) |