1NBS
Crystal structure of the specificity domain of Ribonuclease P RNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9475,0.9293 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 126.500, 145.300, 144.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 3.150 |
| R-factor | 0.28179 |
| Rwork | 0.280 |
| R-free | 0.30700 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.290 * |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 3.240 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.076 * | 0.272 * |
| Total number of observations | 231005 * | |
| Number of reflections | 23276 | |
| <I/σ(I)> | 7.8 | 2.7 |
| Completeness [%] | 98.2 | 80.9 |
| Redundancy | 10.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 30 * | isopropanol, strontium chloride, magnesium chloride, spermine, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 5 (mg/ml) | |
| 2 | 1 | reservoir | isopropanol | 10.5 (%(v/v)) | |
| 3 | 1 | reservoir | 80 (mM) | ||
| 4 | 1 | reservoir | 5 (mM) | ||
| 5 | 1 | reservoir | spermine | 1.5 (mM) | |
| 6 | 1 | reservoir | MES | 50 (mM) | pH6.0 |
