1NBC

BACTERIAL TYPE 3A CELLULOSE-BINDING DOMAIN

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	C 1 2 1
Unit cell lengths	63.570, 50.320, 95.960
Unit cell angles	90.00, 99.47, 90.00

Refinement procedure

Resolution	10.000 - 1.750
R-factor	0.193
Rwork	0.193
R-free	0.25000
Structure solution method	MIRAS
RMSD bond length	0.010
RMSD bond angle	28.670 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000
High resolution limit [Å]	1.750	1.750 *
Rmerge	0.082
Total number of observations	113902 *
Number of reflections	26267
Completeness [%]	87.4	51 *
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5 *	12 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	4 (mg/ml)
2	1	1	MES	25 (mM)
3	1	1	sodium acetate	50 (mM)
4	1	1	PEG3350	12.5 (%)
5	1	2	MES	50 (mM)
6	1	2	sodium acetate	100 (mM)
7	1	2	PEG3350	25 (%)