1NBC
BACTERIAL TYPE 3A CELLULOSE-BINDING DOMAIN
Experimental procedure
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.570, 50.320, 95.960 |
| Unit cell angles | 90.00, 99.47, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.750 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.25000 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 28.670 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | |
| High resolution limit [Å] | 1.750 | 1.750 * |
| Rmerge | 0.082 | |
| Total number of observations | 113902 * | |
| Number of reflections | 26267 | |
| Completeness [%] | 87.4 | 51 * |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 * | 12 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 4 (mg/ml) | |
| 2 | 1 | 1 | MES | 25 (mM) | |
| 3 | 1 | 1 | sodium acetate | 50 (mM) | |
| 4 | 1 | 1 | PEG3350 | 12.5 (%) | |
| 5 | 1 | 2 | MES | 50 (mM) | |
| 6 | 1 | 2 | sodium acetate | 100 (mM) | |
| 7 | 1 | 2 | PEG3350 | 25 (%) |
