1NB8
Structure of the catalytic domain of USP7 (HAUSP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-04-30 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.647, 68.512, 76.261 |
Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.23 |
Rwork | 0.222 |
R-free | 0.27900 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.510 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.057 * | 0.370 * |
Number of reflections | 32867 | |
Completeness [%] | 96.4 | 93.3 |
Redundancy | 3.54 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 * | 295 | PEG 1000, Tris, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | citrate | 100 (mM) | pH5.5 |
3 | 1 | reservoir | PEG3000 | 20 (%) |