1NB0
Crystal Structure of Human Riboflavin Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-25 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0080 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 57.096, 57.096, 82.505 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 * - 1.700 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.22000 * |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.610 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.036 * | 0.200 * |
| Total number of observations | 212199 * | |
| Number of reflections | 16996 * | |
| <I/σ(I)> | 49.2 | 4.3 |
| Completeness [%] | 95.9 | 83.2 |
| Redundancy | 12.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 20 * | NA cadodalyte, Mg acetate, PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-17 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 300 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | pH7.2-7.4 |
| 5 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5 |
| 6 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 23 (%) |
