1NAW
ENOLPYRUVYL TRANSFERASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1995-11-27 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 86.900, 155.900, 83.850 |
Unit cell angles | 90.00, 91.65, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
Rwork | 0.197 |
R-free | 0.27000 |
Structure solution method | MIRAS |
RMSD bond length | 0.016 |
RMSD bond angle | 0.052 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.053 | 0.290 |
Total number of observations | 744941 * | |
Number of reflections | 103189 | |
Completeness [%] | 99.8 | 98.7 |
Redundancy | 7.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | CRYSTALS WERE GROWN BY THE HANGING DROP VAPOR DIFFUSION METHOD USING PLASTIC TISSUE CULTURE PLATES. 0.4 M SODIUM/POTASSIUM PHOSPHATE BUFFER (PH 6.4) CONTAINING 40 MM CYCLOHEXYLAMMONIUM PHOSPHATE WERE EQUILIBRATED AGAINST 1 ML 0.8 M SODIUM/POTASSIUM PHOSPHATE BUFFER (PH 6.4). CRYSTALLIZATION USUALLY OCCURRED WITHIN 3 DAYS AND THE CRYSTALS REACHED THEIR MAXIMUM SIZE OF 0.5 X 0.5 X 0.1 MM==3== AFTER 5 DAYS AT ROOM TEMPERATURE. (SEE REFERENCE 1 FOR DETAILS.), vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | sodium/potassium phosphate | 0.8 (M) | |
2 | 1 | drop | ctclohexylammonium phosphate | 40 (mM) | |
3 | 1 | reservoir | sodium/potassium phosphate | 0.8 (M) |