1NAP
THE CRYSTAL STRUCTURE OF RECOMBINANT HUMAN NEUTROPHIL-ACTIVATING PEPTIDE-2 (M6L) AT 1.9-ANGSTROMS RESOLUTION
Experimental procedure
Spacegroup name | P 1 |
Unit cell lengths | 40.770, 43.810, 44.650 |
Unit cell angles | 98.37, 120.28, 92.78 |
Refinement procedure
Resolution | 7.000 - 1.900 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.23000 |
RMSD bond length | 0.020 |
RMSD bond angle | 0.038 |
Phasing software | X-PLOR |
Refinement software | GPRLSA |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 7.000 * | |
High resolution limit [Å] | 1.900 * | 1.900 * |
Rmerge | 0.025 * | |
Total number of observations | 30511 * | |
Number of reflections | 17830 * | |
Completeness [%] | 97.0 * | 47 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 * | 22 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 17 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 28 (%) | |
3 | 1 | reservoir | sodium acetate | 100 (mM) | |
4 | 1 | reservoir | ammonium acetate | 200 (mM) |