1NAM
MURINE ALLOREACTIVE SCFV TCR-PEPTIDE-MHC CLASS I MOLECULE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 101.860, 101.860, 201.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.700 |
| R-factor | 0.237 * |
| Rwork | 0.230 |
| R-free | 0.29700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fo0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.100 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.083 * | 0.437 * |
| Number of reflections | 29887 | |
| <I/σ(I)> | 7.9 | 1.6 |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG6000 13-17%, MgAc 0.1M, NaCl 0-0.1M, Hepes 0.1M, pH 7.0 to 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 13-17 (%) | |
| 3 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0-7.5 |
| 4 | 1 | reservoir | magnesium acetate | 0-0.1 (M) | |
| 5 | 1 | reservoir | 0-0.1 (M) |
