1NAG

CREVICE-FORMING MUTANTS IN THE RIGID CORE OF BOVINE PANCREATIC TRYPSIN INHIBITOR: CRYSTAL STRUCTURES OF F22A, Y23A, N43G, AND F45A

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	55.720, 37.980, 23.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.163
Rwork	0.163
RMSD bond length	0.013
RMSD bond angle	0.036 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.850 *
Rmerge	0.058 *
Number of reflections	4421 *
Completeness [%]	98.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	9 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein solution	8 (mg/ml)
2	1	drop	potassium phosphate	0.6 (M)
3	1	reservoir	potassium phosphate	1.2-1.3 (M)