1NAF
Crystal structure of the human GGA1 GAT domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-15 |
Wavelength(s) | 0.9796, 0.9798, 0.9393 |
Spacegroup name | P 63 |
Unit cell lengths | 82.700, 82.700, 69.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.2484 |
Rwork | 0.248 |
R-free | 0.28300 * |
Structure solution method | MAD |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.180 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.066 | 0.399 |
Number of reflections | 6684 | |
<I/σ(I)> | 14.2 | 2.6 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 4.5 | 289 | sodium acetate, lithium sulphate, NaH2PO4/K2HPO4, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | dithiothreitol | 5 (mM) | |
2 | 1 | drop | protein | 10-15 (mg/ml) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.5 |
4 | 1 | reservoir | sodium phosphate | 0.3 (M) | |
5 | 1 | reservoir | potassium phosphate | 0.45 (M) | |
6 | 1 | reservoir | 0.2 (M) |