1NA6
Crystal structure of restriction endonuclease EcoRII mutant R88A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2002-04-10 |
Detector | CUSTOM-MADE |
Wavelength(s) | 1.0088 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.746, 92.360, 88.308 |
Unit cell angles | 90.00, 108.08, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.236 |
Rwork | 0.230 |
R-free | 0.28400 |
Structure solution method | SIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | ISIRAS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.053 | 0.387 |
Total number of observations | 259113 * | |
Number of reflections | 52027 * | |
<I/σ(I)> | 11.5 | 3.8 |
Completeness [%] | 99.6 * | 99.9 * |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | cacodylate | 100 (mM) | pH6.3-6.5 |
2 | 1 | reservoir | methanol | 3.5-4 (%(v/v)) | |
3 | 1 | reservoir | 35-40 (mM) |