1NA3
Design of Stable alpha-Helical Arrays from an Idealized TPR Motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.986, 54.941, 66.938 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.700 * - 1.550 |
| R-factor | 0.18102 |
| Rwork | 0.178 |
| R-free | 0.20700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.500 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.700 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.042 | 0.860 |
| Number of reflections | 22285 | |
| <I/σ(I)> | 43.3 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | Tris, PEG 4000, MgCl2, IPTG, NaCl, pH 8., VAPOR DIFFUSION, HANGING DROP at 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | Tris | 100 (mM) | pH8. |
| 5 | 1 | reservoir | PEG4000 | 40 (%) | |
| 6 | 1 | reservoir | 0.1 (M) | ||
| 7 | 1 | reservoir | IPTG | 10 (%) |
