1N9Z
INTEGRIN ALPHA M I DOMAIN MUTANT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-02-08 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.542 |
Spacegroup name | P 43 |
Unit cell lengths | 44.220, 44.220, 99.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.247 |
Rwork | 0.242 |
R-free | 0.27700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ido |
RMSD bond length | 0.013 |
RMSD bond angle | 1.710 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.660 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.111 * | 0.347 * |
Total number of observations | 14631 * | |
Number of reflections | 6234 * | |
Completeness [%] | 95.5 | 97.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 23 * | PEG 3000, sodium chloride, hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 100 (mM) | ||
2 | 1 | drop | 5 (mM) | ||
3 | 1 | drop | Tris | 20 (mM) | pH8.0 |
4 | 1 | drop | protein | 10 (mg/ml) | |
5 | 1 | reservoir | 0.2 (M) | ||
6 | 1 | reservoir | PEG3000 | 20 (%) | |
7 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |