1N9Z

INTEGRIN ALPHA M I DOMAIN MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-02-08
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.542
Spacegroup name	P 43
Unit cell lengths	44.220, 44.220, 99.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.247
Rwork	0.242
R-free	0.27700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ido
RMSD bond length	0.013
RMSD bond angle	1.710 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.660
High resolution limit [Å]	2.500	2.500
Rmerge	0.111 *	0.347 *
Total number of observations	14631 *
Number of reflections	6234 *
Completeness [%]	95.5	97.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8 *	23 *	PEG 3000, sodium chloride, hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		100 (mM)
2	1	drop		5 (mM)
3	1	drop	Tris	20 (mM)	pH8.0
4	1	drop	protein	10 (mg/ml)
5	1	reservoir		0.2 (M)
6	1	reservoir	PEG3000	20 (%)
7	1	reservoir	HEPES	0.1 (M)	pH7.5